Conceptual Framework of Adrenal Stress Index
_________________________________________________________

Test Specifications

ADRENAL STRESS INDEX

The Adrenal Stress Index panel includes four tests.
Complete Panel is perforomed using four timed saliva samples.
Sample stability for all tests is a minimum of 14 days at room temperature.
Name of Test Temporal Adrenal Profile
Specimen Saliva: 4 collections at specified time intervals.
Tests Free Fraction of Cortisol
Patient Prepartion Refer to request form for collection instructions.
Reference Intervals Normal Adults: Circadian Rhythm values, Figure 1.
7:00-8:00 AMxxxxxxxxxx13-23 nM
Noon-1:00 PMxxxxxxxxxx4-8 nM
3:30-4:30 PMxxxxxxxxxxx4-8 nM
11:00-Midnightxxxxxxxxx 1-3 nM
60 min After ACTHxxxxx40-80 nM
Insulin Challenge Testxxxx 8-20 nM
Desamethasone Suppression Test, DST:<1nM
Hypercortisol:> 24nM (any time) or Cortisol Time Integral > 80
Adrenal Insufficiency: Reduced Circadian Rhythm values see above.
Addison's Disease:<1nM (any time)
Name of Test DHEA & DHEA(S) pooled value
Specimen Saliva: 2 collection at noon and afternoon (when performed alone).
Patient Preparation Refer to request form for collection instructions.
Test Performance Salivary DHEA & DHEA(S) correlate >90% with circulating levels.
Test Limitations Not suitable for individuals with bleeding gums and overt gingivitis.
Reference Intervals 3-10 ng/ml - Adults all ages.

Test Specifications (continued)
Name of Test Total Secretory IgA (SIgA)
Specimen Saliva: 1 specimen (when performed alone).
Patient Preparation Refer to request form for collection instructions.
Test Limitations Not suitable for individuals with bleeding gums and overt gingivitis.
Reference Intervals 25-60 mg/dL Normal
20-25 mg/dL Borderline
<20 mg/dL Depressed
Name of Test AntiGliadin Antibody, IgA Fraction
Specimen Saliva: 1 specimen (when performed alone).
Patient Preparation Refer to request form for collection instructions.
Test Limitations Not suitable for individuals with bleeding gums and overt gingivitis.
Reference Intervals >15 IU/mL Positive
13-15 IU/mL Borderline
<13 IU/mL Negative

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6620 South 192nd Place, Building J
Kent, Washington 98032
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